Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine.
نویسندگان
چکیده
One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.
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ورودعنوان ژورنال:
- Analytical biochemistry
دوره 132 2 شماره
صفحات -
تاریخ انتشار 1983